A Study on the effect of Chemical and Enzymatic Factors on the Solubility of Shrimp Head Waste ProteinsMizani, M; Aminlari, M; Khodabandeh, M; 2004; A Study on the effect of Chemical and Enzymatic Factors on the Solubility of Shrimp Head Waste Proteins; Journal of Agricultural Sciences, Vol. 10, No. 2, 59-72.
Abstract: Shrimp head waste (SHW) has been studied as a valuable source of protein (64.1%, dwb). In the present research the effect of different factors including pH, chemical agents such as NaCl, NaSO3, Triton x-100 and also application of commercial proteolytic enzyme, Alcalase, on solubility of SHW proteins and electrophoresis patterns of the extracted proteins was investigated. The results show that pH=8 is the optimum for protein extraction. Three of the factors (i.e. NaSO3, Triton x-100 and Alcalase) improve the results successfully, at their optimum concentration (200mmol/l, 1% and 0.5%) respectively. Enzymatic treatment, using Alcalase, alone or in combination with NaSO3 /or Triton x-100 resulted in higher solubilization of proteins. SDS-PAGE patterns revealed appearance of small molecular weight proteins, less than 14-20 kDa, due to chemical or enzymatic treatments, which probably contribute to the higher solubility. These results indicate that it is possible to improve protein solubility by treatment with NaSO3 , Alcalase or in combination of both.
Keywords: Shrimp Head Waste, Alcalase, Protein Extraction.
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Abstract: Shrimp head waste (SHW) has been studied as a valuable source of protein (64.1%, dwb). In the present research the effect of different factors including pH, chemical agents such as NaCl, NaSO3, Triton x-100 and also application of commercial proteolytic enzyme, Alcalase, on solubility of SHW proteins and electrophoresis patterns of the extracted proteins was investigated. The results show that pH=8 is the optimum for protein extraction. Three of the factors (i.e. NaSO3, Triton x-100 and Alcalase) improve the results successfully, at their optimum concentration (200mmol/l, 1% and 0.5%) respectively. Enzymatic treatment, using Alcalase, alone or in combination with NaSO3 /or Triton x-100 resulted in higher solubilization of proteins. SDS-PAGE patterns revealed appearance of small molecular weight proteins, less than 14-20 kDa, due to chemical or enzymatic treatments, which probably contribute to the higher solubility. These results indicate that it is possible to improve protein solubility by treatment with NaSO3 , Alcalase or in combination of both.
Keywords: Shrimp Head Waste, Alcalase, Protein Extraction.
click on image for large view
Visit: 2872
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